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Guanidinium thiocyanate - Wikipedia
Guanidinium thiocyanate or guanidinium isothiocyanate (GITC) is a chemical compound used as a general protein denaturant, being a chaotropic agent, although it is most commonly used as a nucleic acid protector in the extraction of DNA and RNA from cells. GITC may also be recognized as guanidine …
SDS SAFETY DATA SHEET - puritanproducts
GUANIDINE THIOCYANATE Page 1 of 6 Effective Date: 05/10/17 Initial Release NON-EMERGENCY TELEPHONE 24-HOUR CHEMTREC EMERGENCY TELEPHONE 610-866-4225 800-424-9300 SDS – SAFETY DATA SHEET 1. Identification Product Identifier: GUANIDINE THIOCYANATE Synonyms: Guanidine Isothiocyanate Chemical Formula: NH2C(NH) NH2 HSCN
Guanidine Thiocyanate | CAS 593-84-0 | Protein Denaturant .
Description. Guanidine thiocyanate is a compound that is widely used for the isolation and extraction of RNA in biochemical research. In this capacity, it has been used in many studies relating to cell function, signaling and gene expression.Guanidine thiocyanate can also be used to create whole blood lysates for long-term storage or transportation of samples.
Guanidine thiocyanate solution | Sigma-Aldrich
Sigma-Aldrich offers a number of Guanidine thiocyanate solution products. View information & documentation regarding Guanidine thiocyanate solution, including CAS, MSDS & more.
SAFETY DATA SHEET - nwmissouri.edu
(M)SDS sections updated. 16. Other information For research use only. U.S. Federal Regulations This product has been classified according to the hazard criteria of the CPR and the MSDS contains all of the information required by the CPR Component Component CAS-No Weight % HAPS data Guanidine isothiocyanate 593-84-0 ( 15-40 ) Phenol 108-95-2 .
Lysis Buffer | Bio-Rad
Cell lysis buffer for RNA extraction is highly denaturing and is usually composed of phenol and guanidine isothiocyanate. RNase inhibitors are usually present in the lysis buffer, since RNases can be very resistant to denaturation and remain active. For extraction of DNA the lysis buffer will commonly contain SDS.
Guanidine Isothiocyanate - Thermo Fisher Scientific
UltraPure Guanidine Isothiocyanate is a strong protein denaturant when used at high concentrations. It is useful in the isolation of intact RNA from sources rich in RNase such as pancreas (1).
SAFETY DATA SHEET - Fisher Scientific
Synonyms Guanidinium isothiocyanate; Thiocyanic acid, compound with Guanidine (1:1) Recommended Use Laboratory chemicals. Uses advised against Food, drug, pesticide or biocidal product use Details of the supplier of the safety data sheet Emergency Telephone Number For information US call: 001-800-ACROS-01 / Europe call: +32 14 57 52 11
A Unique Method for Isolation and Solubilization of .
This protein precipitate was washed thrice with 0.3 M guanidine hydrochloride in 95% ethanol. Further, the protein precipitate was vacuum-dried and dissolved in 1% SDS. To ensure maximum dissolution, the precipitate, along with 1% SDS, was incubated at 50°C for longer than the recommended time of 10 min with intermittent vortexing.
Guanidine Thiocyanate - Thermo Fisher Scientific
Guanidine Thiocyanate is an ultrapure, molecular biology grade reagent. It is free of detectable nuclease and protease activity and is a strong protein denaturant, as both the guanidinium cation and the thiocyanate anion are chaotropic agents. It is provided in one bottle containing 500 g. It is rec
Protein Denaturation: Urea vs Guanidine Hydrochloride (GdnHCl)
Protein Denaturation: Urea vs Guanidine Hydrochloride (GdnHCl) In equilibrium denaturation experiments, the concentration of urea at mid-point of denaturation …
Guanidine thiocyanate for molecular biology, ≥99% | Sigma .
Guanidine thiocyanate is a denaturing agent and is used routinely in RNA isolation. It is used as a storage buffer for whole blood samples. Guanidine thiocyanate inactivates nucleases and is ideal for storing and freezing fecal samples for DNA studies. It is used in combination with phenol−chloroform in …
Guanidine thiocyanate, Ultrapure - TOKU-E
Guanidine thiocyanate can be used to isolate RNA from both cell culture and tissue form human and animal sources. Authors extracted a protocol for extracting RNA from human placental tissue and from primary and transformed cell lines of human and animal origin, without any ultracentrifugation steps or phenol extractions.
Guanidine Thiocyanate CAS 593-84-0
Stronger chaotropic agent than Guanidine Hydrochloride. Used in total RNA preparation as 'guanidinium solution'. Guanidine Thiocyanate is used as a common protein denaturant in the purification and extraction of mRNA, nucleic acids and proteins from cellular material, acting as a strong RNase inhibitor, protecting cellular transcripts from degradation during tissue extraction.
Guanidine Thiocyanate | CAS 593-84-0 | SCBT - Santa Cruz .
Buy Guanidine Thiocyanate (CAS 593-84-0), a useful tool in for RNA isolation and protein solubilization from Santa Cruz. Purity >99%, MF CH5N3•CHNS
Safety Data Sheet - Thermo Fisher Scientific
Guanidine isothiocyanate 593-84-0 ( 15-40 ) 593-84-0 209-812-1 15-40 Ammonium thiocyanate 1762-95-4 ( 7-13 ) 1762-95-4 217-175-6 7-13 Contact with acids or bleach liberates toxic gases. DO NOT ADD acids or bleach to any liquid wastes containing this product. We recommend handling all chemicals with caution. SECTION 4: First aid measures
Safety Data Sheet Guanidine Thiocyanate Section 1 .
Page 1 of 4 Safety Data Sheet Guanidine Thiocyanate Section 1 - Chemical Product and Company Identification 1.1 Product Identifiers: Product H314Name: Guanidine Thiocyanate Catalog Number(s): GT3201, GT3220, GT4205, GT4220, GT5201, GT5202, GT5203, GT5220
RNA extraction - OpenWetWare
Guanidine isothiocyanate extraction coupled with lithium chloride precipitation. Lyses cells, extracts cellular RNA and denatures proteins all at once in many cases. Inactivates RNases faster than acid phenol extraction. Does not physically separate RNA from proteins and DNA in one step.
Guanidine Thiocyanate at Thomas Scientific
Guanidine Thiocyanate found in: Guanidine thiocyanate, Guanidine Thiocyanate, Guanidine thiocyanate, =97% (titration), Guanidine Thiocyanate, Guanidine.. . Also known as Guanidine isothiocyanate Biotechnology Grade A280 (70%, water, 250°C): <0.8 . • The denatured protein is suitable for SDS-PAGE and western blotting.
593-84-0・グアニジンチオシアン・Guanidine Thiocyanate …
Guanidine Thiocyanate, Molecular Grade (Guanidinium .
Guanidine Thiocyanate, Molecular Grade, at high concentrations, is a protein denaturant used most commonly for the isolation of intact RNA due to its ability to inhibit RNase. Formula Weight: 118.16. Form: White, crystalline powder.
Rapid extraction of DNA from diverse soils by guanidine .
Rapid extraction of DNA from diverse soils by guanidine thiocyanate method Anupriya Agarwal*, . guanidine isothiocyanate were employed. In the . Na2EDT A, (pH 8.0), 4% SDS and 500 jll of 8 M guanidine thiocyanate (SRL Pvt. Ltd., Bombay) was added to …
Material Safety Data Sheet
Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid imme diately. Skin: Get medical aid. Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes.